Susan Strome Wood, Wb
Published in
Proceedings of the National Academy of Sciences
By using fluorescent antibody staining, we have followed cytoplasmic granules unique to germ-line cells throughout the life cycle of Caenorhabditis elegans. These elements, designated P granules, are segregated exclusively to germ-line precursor cells during early embryogenesis. Prior to mitosis at each of the early cleavages that produce a somatic...
Yager, J Richards, S Hekmat-Scafe, Ds Hurd, Dd Sundaresan, V Caprette, Dr Saxton, Wm Carlson, Jr Stern, M
Published in
Proceedings of the National Academy of Sciences
Drosophila peripheral nerves, similar structurally to the peripheral nerves of mammals, comprise a layer of axons and inner glia, surrounded by an outer perineurial glial layer. Although it is well established that intercellular communication occurs among cells within peripheral nerves, the signaling pathways used and the effects of this signaling ...
Knutson, Ak Rechtsteiner, A Susan Strome
Published in
Proceedings of the National Academy of Sciences
The germ lineage is considered to be immortal. In the quest to extend lifespan, a possible strategy is to drive germ-line traits in somatic cells, to try to confer some of the germ lineage s immortality on the somatic body. Notably, a study inCaenorhabditis eleganssuggested that expression of germ-line genes in the somatic cells of long-liveddaf-2m...
Saxton, Wm Porter, Me Cohn, Sa Scholey, Jm Raff, Ec Mcintosh, Jr
Published in
Proceedings of the National Academy of Sciences
Preparations of kinesin, a microtubule-based force-producing protein, have been isolated from Drosophila melanogaster embryos by incubation of microtubules with a nonhydrolyzable ATP analogue and gel filtration of proteins released from the microtubules by ATP. These preparations induced MgATP-dependent microtubule gliding in vitro with a Km for Mg...
Zhang, C Song, Y Thompson, Da Madonna, Ma Glenn Millhauser Toro, S Varga, Z Westerfield, M Gamse, J Chen, W
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Published in
Proceedings of the National Academy of Sciences
Background adaptation is used by teleosts as one of a variety of camouflage mechanisms for avoidance of predation. Background adaptation is known to involve light sensing by the retina and subsequent regulation of melanophore dispersion or contraction in melanocytes, mediated by α-melanocyte-stimulating hormone and melanin-concentrating hormone, re...
Michael Stone Bryant, Z Crisona, Nj Smith, Sb Vologodskii, A Bustamante, C Cozzarelli, Nr
Published in
Proceedings of the National Academy of Sciences
Escherichia coli topoisomerase (Topo) IV is an essential type II Topo that removes DNA entanglements created during DNA replication. Topo IV relaxes (+) supercoils much faster than (-) supercoils, promoting replication while sparing the essential (-) supercoils. Here, we investigate the mechanism underlying this chiral preference. Using DNA binding...
Zu, Tao Gibbens, Brian Doty, Noelle S Gomes-Pereira, Mário Huguet, Aline Stone, Matthew D Margolis, Jamie Peterson, Mark Markowski, Todd W Ingram, Melissa A C
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Published in
Proceedings of the National Academy of Sciences of the United States of America
Trinucleotide expansions cause disease by both protein- and RNA-mediated mechanisms. Unexpectedly, we discovered that CAG expansion constructs express homopolymeric polyglutamine, polyalanine, and polyserine proteins in the absence of an ATG start codon. This repeat-associated non-ATG translation (RAN translation) occurs across long, hairpin-formin...
Djurisic, M. Vidal, G. S. Mann, M. Aharon, A. Kim, T. Ferrao Santos, A. Yi Zuo Hubener, M. Shatz, C. J.
Published in
Proceedings of the National Academy of Sciences
Glenn Millhauser Salpeter, Ee Oswald, Re
Published in
Proceedings of the National Academy of Sciences
The lifetimes of the unitary currents from ion channels, as revealed from single-channel recording, are traditionally thought to follow exponential or multiexponential distributions. The interpretation of these event-time distributions is that the gating process follows Markov kinetics among a small number of states. There is recent evidence, howev...
Jeremy Sanford Ellis, Jd Cazalla, D Cáceres, Jf
Published in
Proceedings of the National Academy of Sciences
The Ser/Arg-rich (SR) proteins constitute a family of highly conserved nuclear phosphoproteins that are involved in many steps of mRNA metabolism. Previously, we demonstrated that shuttling SR proteins can associate with translating ribosomes and enhance translation of reporter mRNAs both in vivo and in vitro. Here, we show that endogenous, cytopla...
